Immunodiagnosis of Human Toxocariasis
نویسندگان
چکیده
Human toxocariasis is a helminthic zoonosis caused by larval stages of Toxocara canis and, less frequently, by T. cati, the roundworms of dogs and cats, respectively. Accidentally, humans ingest embryonated eggs containing the infective larva which are released in the upper small intestine and then pass through the intestinal epithelium to reach the blood vessels, where they can migrate to the different visceral organs and tissues of the body (Despommier, 2003).
منابع مشابه
Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis.
In the present work, we identified adult Toxocara canis antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other parasitic infections, were assessed by IgM and IgG-enzime linked immunosorbent assay. Whilst we f...
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A dot enzyme-linked immunosorbent assay (dot-ELISA) was standardized using excretory-secretory antigens of Toxocara canis for the rapid immunodiagnosis of human toxocariasis. Thirty patients with clinical signs of toxocariasis, 20 cases with other parasitic diseases, and 40 healthy subjects were tested. A total of 0.2 ng of antigen per dot, serum dilution of 1:160 and dilution conjugate of 1:10...
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The specificity of the recombinant Toxocara canis antigen developed for the immunodiagnosis of human toxocariasis was compared with that of the excretory-secretory antigen from T. canis second-stage larvae (TES) by enzyme-linked immunosorbent assay. A total of 153 human serum samples from patients infected with 20 different helminths, including 11 cases of toxocariasis, were examined. No false-...
متن کاملTrend of toxocariasis in Iran: a review on human and animal dimensions
One of the neglected soil and/or food-borne diseases with international public health importance is toxocariasis. Human cases are being increasingly reported from Asian, African, Oceania, European and the American countries. Hence, human toxocariasis (HT) is now considered as a major zoonosis with global and regional importance. In Iran, human and animal toxocariasis is an endemic disease with ...
متن کاملتخلیص و ارزیابی آنتی ژنهای اصلی مایع هیداتیک در تشخیص ایمونولوژیک هیداتیدوز
Regarding remarkable prevalence of human hydatidosis and despite introduction of novel sensitive techniques such as ELISA for immunodiagnosis of disease in the word, there has not been much progress in that field in Iran. Preparation and purification of suitable antigens is the basic and first step in serodiagnosis of this disease. The present study, thus, is designed and carried out for pr...
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